IN VITRO ORCHID CULTURE MEDIA

IN VITRO ORCHID CULTURE MEDIA

VACIN & WENT MINERAL SALT FORMULATION (Russell Modification)

(This formulation does away with the use of the barely soluble Tri-calcium Phosphate for which the original formulation calls, yet gives identical ion concentrations to the original. The Iron-source has additionally been replaced with a commercial chelated form of horticultural iron.)

CALCIUM NITRATE	Ca(NO₃)2.4H2O	450 mg
POTASSIUM NITRATE	KNO₃	140 mg
MONO-POTASSIUM PHOSPHATE	KH₂PO4	80 mg
DI-POTASSIUM PHOSPHATE	K₂HPO4	440 mg
MAGNESIUM SULPHATE	MgSO₄.7H2O	250 mg
AMMONIUM SULPHATE	(NH₄)2SO4	500 mg
MANGANESE SULPHATE	MnSO₄	7.5 mg
13% IRON CHELATE		40 mg
DISTILLED WATER		to 1 litre

MOTHER FLASK MEDIUM - FOR GERMINATION OF SEED

V & W SALTS
SUCROSE		20 g
AGAR		8 g

pH ADJUSTED TO Ca. 6.2 WITH CITRIC ACID (Ca. 75 mg) OR LEMON JUICE (Ca. 1 ml)

GROWTH MEDIUM - FOR REFLASKING SEEDLINGS

V & W SALTS
SUCROSE		20 g
AGAR		6 g
PEPTONE		1 g
BANANA		Ca. 50 g
NAA		1 mg

pH ADJUSTED AS ABOVE

SIMPLE MEDIUM

CHEMICULT HYDROPONIC FERTILIZER		2 g
SUCROSE (TABLE SUGAR)		20 g
AGAR		8 g
DISTILLED WATER		to 1 litre

ABOVE MEDIA ARE SUITABLE FOR EPIPHYTIC AND SEMI-EPIPHYTIC ORCHIDS, SUCH AS ANSELLIA, CYMBIDIUM, CATTLEYA, DENDROBIUM, ONCIDIUM, etc. FOR GENERA LIKE EULOPHIA, PAPHIOPEDILUM, THE DISA GROUP, etc., MORE COMPLICATED MEDIA HAVE TO BE USED.

© GREIG RUSSELL 7.11.92

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